The use of spin desalting columns in DMSO-quenched H/D-exchange NMR experiments
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چکیده
منابع مشابه
The use of spin desalting columns in DMSO-quenched H/D-exchange NMR experiments.
Dimethylsulfoxide (DMSO)-quenched hydrogen/deuterium (H/D)-exchange is a powerful method to characterize the H/D-exchange behaviors of proteins and protein assemblies, and it is potentially useful for investigating non-protected fast-exchanging amide protons in the unfolded state. However, the method has not been used for studies on fully unfolded proteins in a concentrated denaturant or protei...
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We studied hydrogen/deuterium-exchange reactions of peptide amide protons of GroES using two different techniques: (1) two-dimensional (1)H-(15)N transverse-optimized NMR spectroscopy and (2) the dimethylsulfoxide-quenched hydrogen-exchange method combined with conventional (1)H-(15)N heteronuclear single quantum coherence spectroscopy. By using these techniques together with direct heteronucle...
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چکیده ندارد.
15 صفحه اولDiafiltration for Desalting or Buffer Exchange
Diafiltration is an ultrafiltration membrane technique for completely removing, replacing, or lowering the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids, and other biomolecules. The process selectively uses permeable (porous) membrane filters to separate the components of solutions and suspensions based on their molecular size. Smaller molecules ...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2013
ISSN: 0961-8368
DOI: 10.1002/pro.2221